77 Batson Drive Manchester, CT 06042 Phone: (860) 643-9560 Email: NEB@gza.com

Organism Culturing

 

New England Bioassay recognizes that our client's samples must be tested using only healthy stocks of freshwater, saltwater, or terrestrial organisms. Our experienced staff maintains and cultures a wide variety of test species. Before testing begins, much effort is expended on the preparation of our organisms.
     
Organisms used in testing must be grown and then acclimated to our client's test-specific requirements, such as life stage, age, water type, and temperature regime.

NEB routinely maintains large breeding stocks of Fathead minnows (Pimephales promelas), to ensure a ready supply of eggs and fry for testing. In-house freshwater invertebrate cultures include the Daphnids Daphnia pulex , Daphnia magna, and Ceriodaphnia dubia). For sediment toxicological investigations, NEB also cultures amphipods, Hyalella azteca, and chironomids, Chironomus dilutus. Axenic stocks of the freshwater alga, Pseudokirchneriella subcapita (formerly Selenastrum capricornutum), are maintained as a food source for daphnids and as test organisms in algal growth and inhibition tests.

Saltwater in-house invertebrate cultures include the mysid shrimp (Americamysis bahia (formerly Mysidopsis) and the amphipod Leptocheirus plumulosus. Facilities and equipment are also available for long-term holding of the sea urchin (Arbacia punctulata), inland silversides (Menidia beryllina), sheepshead minnows (Cyprinodon variegatus), the amphipod Ampelisca abdita, the clam Macoma nasuta and the worm Nereis virens.

When demand for test organisms exceeds laboratory production, reputable commercial suppliers are used. When using organisms from an outside source, US EPA guidelines for holding and acclimating organisms are followed. For all organisms used in toxicological investigations, reference toxicant tests [e.g., copper (nitrate & sulfate), sodium dodecyl sulfate, potassium chloride, and sodium chloride] are routinely performed to assess organism health. For in-house cultured organisms, health is verified at least once per month. For organisms obtained from commercial suppliers, reference toxicant tests are performed to assess organism health. NEB maintains internally-generated cumulative-summation control charts for both cultured and purchased test organisms.

Learn more about our test species on the following pages:

Freshwater Species

Saltwater Species

Sediment & Soil Species