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Wastewater Test Methods

The EPA publishes laboratory test procedures that are used to analyze the chemical, physical and biological components of wastewater and other environmental samples that are required by regulations under the authority of the Clean Water Act (CWA).  The following table lists the most commonly used Whole Effluent Toxicity (WET) Testing Methods, all of which our laboratory can provide.

 

FRESHWATER ACUTE TOXICITY TEST METHODS

TEST METHOD 2002.0: Daphnid, Ceriodaphnia dubia
TEST METHOD 2021.0: Daphnid, Daphnia pulex and Daphnia magna
TEST METHOD 2000.0: Fathead Minnow, Pimephales promelas
TEST METHOD 2019.0: Rainbow Trout Oncorhynchus mykiss and Brook Trout Salvelinus fontinalis

FRESHWATER CHRONIC TOXICITY TEST METHODS

TEST METHOD 1002.0: Daphnid, Ceriodaphnia dubia , Survival & Reproduction
TEST METHOD 1000.0: Fathead minnow, Pimephales promelas , Larval Survival & Growth
TEST METHOD 1003.0: Green Alga, Selenastrum capricornutum, Growth

SALTWATER ACUTE TOXICITY TEST METHODS

TEST METHOD 2007.0: Mysid, Americamysis bahia (formerly Mysidopsis )
TEST METHOD 2004.0: Sheepshead Minnow, Cyprinodon variegatus
TEST METHOD 2014.0: Silverside, Menidia beryllina, M. menidia , and M. peninsulae

SALTWATER CHRONIC TOXICITY TEST METHODS

TEST METHOD 1007.0: Mysid, Mysidopsis bahia , Survival, Growth, & Fecundity
TEST METHOD 1004.0: Sheepshead Minnow, Cyprinodon variegatus , Larval Survival & Growth
TEST METHOD 1006.0: Inland Silverside, Menidia beryllina , Larval Survival & Growth
TEST METHOD 1008.0: Sea Urchin, Arbacia punctulata, Fertilization